Thank you, I was so pleased to see this transfer grow out so vigorously, knew I had to share!
For starters, I do all my work under a laminar flow hood. This is from a liquid culture syringe, it took about two weeks for the first blob of mycelium to grow out on the agar and was a bit sporadic but with sections of nice rhizomorphic growth. Once I had enough good sections to choose from, I transferred a few wedges to fresh plates. From there it was about 12 days until I had the plate above, but it was very consistent growth across all transfers (took 3, this is just the one I ended up turning into LC).
For agar, I make my own using this video by Fungaia as a general recipe base. It’s more involved to make because it calls for more than just water/agar/sugar/peptone, but rather all that plus a mix of pulverised grains, straw, hardwoods, and other trace minerals. I strongly believe in it, though, I feel like the industry standard recipe for agar is easier to make but lacks complex nutrition. In the video, it is equated to a person drinking nothing but soda. Sure, there’s calories there that our bodies are capable of processing, but that doesn’t mean you’ll be healthy, especially when compared to a person eating full balanced meals.
For capturing and storing genetics, I like using a tube like this to drop some of the best agar wedges into, top that up with sterilized water, cap, wrap the cap with parafilm, then fridge.
Fungaia’s video on pressure cookers may be very helpful to you! It doesn’t go over the specifics for chicken manure, but goes over pressure cookers in general and is very informative. It also goes over lots of different types (instant pot/presto canner/etc) so you can choose the scale/price range that fits your goals. And besides the PC video, there’s quite a few other diy cultivation videos to watch. Hope something there might be helpful c: Good luck, and mush love!
It’s fledgling, but check out https://www.votelabor.org/ It’s a small but growing group of Americans who want to start a party that advocates for the working class. I jumped in yesterday and am gonna try to do my part. There’s channels for every state to organize amongst their state peers, national channels for overall organization, policy discussions currently under debate, and desire to start getting people on ballots and in seats as soon as possible